ABSTRACT
To assess the hepatoprotective activity of Glutathione S-transferase [GSTsw], extracted and purified from silkworm, in experimental acute mice liver injury and explore mechanisms. Mice were divided into five groups: control group, carbon tetrachloride [CC1[4]] group, and three treatment groups that received CC1[4] and GSTsw at doses of 0.083mg-g[-1], 0.0415 mg-g[-1] and 0.0207 mg-g[-1] for 3 days. ALT in serum, GST, SOD and T-AOC in liver tissue homogenate, and changes in liver pathology in the five groups were studied. CC1[4] administration led to pathological and biochemical evidence of liver injury as compared to untreated controls. GSTsw administration led to significant protection against CCl[4]-induced changes in liver pathology. It was also associated with significantly lower serum ALT levels, higher GST - SOD and T-AOC level in live tissue homogenate. Thus, GSTsw showed protective activity against CCl[4]-induced hepatotoxicity in mice